Films for the Humanities and Sciences - Extraction of DNA from Cells

Extraction of DNA from Cells

This program shows the process of DNA extraction: grinding liver tissue using liquid nitrogen to prevent degradation of cellular contents, adding a lysis buffer, rupturing the cell membrane to liberate cellular contents, and separating the DNA from other cellular components such as protein. After several further steps, the DNA is lifted out, dissolved in a small volume of distilled water, and the concentration of DNA in this solution is estimated by means of a spectrophotometer. The program also shows how restriction enzymes are used to cut the DNA into shorter pieces. (15 minutes)

Tell a Friend!

Your Name:
Your Email:
Your Friend's Email:
Your Message:
We respect your privacy and will not use your friend's address without permission.

Item#: BVL3993
Copyright date: ©1992

Part of the Series : DNA in Practice


DVD	$79.95

Prices include public performance rights.
Only available in the US and Canada.

Related Titles

Origins of Change: Heredity and Mutation

This program introduces the concepts of naturally occurring and artificially induced mutagens, demonstrates how X-radiation and chemical additives can produce genetic mutations, introduces Dr. Maclyn McCarty (one of three researchers who identified D...(more details)

DVD	$99.95
DVD + 3-Year Streaming	$149.93
3-Year Streaming	$99.95

The Physiology of Exercise

Skeletal muscle exercise expends the stored energy of adenosine triphosphate (ATP) derived initially from the oxidation of glucose. Since most regeneration of ATP for prolonged activity is achieved directly from the oxidation of glucose brought to th...(more details)

DVD

$69.95

Gel Electrophoresis

This program follows the procedures for placing DNA in a well at one end of an electrophoresis gel: heating the gel and containing it when molten, leaving it to set, loading the digested DNA samples together with loading buffer, and switching on the ...(more details)

DVD

$79.95

Southern Blotting

To enable us to look at specific genes, the DNA is transferred from the gel onto a membrane made of nitrocellulose or nylon. This process is known as southern blotting. The principle is very simple: the capillary action of liquid passing through the ...(more details)

DVD

$79.95

Hybridization

In order for a gene to be visible, it must be probed with a length of nucleic acid with the same sequence. These probes are labeled with radioactivity. This program follows the procedures as well as the necessary safety precautions until at the end a...(more details)

DVD

$79.95

See additional titles in General Biology | Human Anatomy and Physiology