To enable us to look at specific genes, the DNA is transferred from the gel onto a membrane made of nitrocellulose or nylon. This process is known as southern blotting. The principle is very simple: the capillary action of liquid passing through the gel and the membrane is used to transfer the fragments of DNA; the DNA cannot pass through this membrane and is immobilized on it. In this program, this is the process we observe being carried out. (13 minutes)

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Part of the Series : DNA in Practice

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		The Physiology of Exercise Skeletal muscle exercise expends the stored energy of adenosine triphosphate (ATP) derived initially from the oxidation of glucose. Since most regeneration of ATP for prolonged activity is achieved directly from the oxidation of glucose brought to th...(more details)		DVD $69.95
		Gel Electrophoresis This program follows the procedures for placing DNA in a well at one end of an electrophoresis gel: heating the gel and containing it when molten, leaving it to set, loading the digested DNA samples together with loading buffer, and switching on the ...(more details)		DVD $79.95
		Inheritance in a Fungus This program illustrates the experimental techniques involved in the genetic analysis of an ascospore color mutant in Sordaria. Two self-sterile but interbreeding strains are used. Mutations are induced by ultraviolet irradiation which result in the ...(more details)		DVD $69.95
		Extraction of DNA from Cells This program shows the process of DNA extraction: grinding liver tissue using liquid nitrogen to prevent degradation of cellular contents, adding a lysis buffer, rupturing the cell membrane to liberate cellular contents, and separating the DNA from o...(more details)		DVD $79.95
		Hybridization In order for a gene to be visible, it must be probed with a length of nucleic acid with the same sequence. These probes are labeled with radioactivity. This program follows the procedures as well as the necessary safety precautions until at the end a...(more details)		DVD $79.95

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