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Hybridization

In order for a gene to be visible, it must be probed with a length of nucleic acid with the same sequence. These probes are labeled with radioactivity. This program follows the procedures as well as the necessary safety precautions until at the end an autoradiograph is obtained. The dark bands indicate where the labeled DNA probe has bound to the DNA of the same sequence on the filer. This provides the final result. It is from this apparently simple pattern of bands that the information we need is to be found—the source of the exciting developments in biotechnology. (15 minutes)



 
    

Item#: BVL3996
Copyright date: ©1992



Part of the Series : DNA in Practice
     
DVD $79.95


Prices include public performance rights.
Only available in the US and Canada.





DNA and the Evidence for Evolution
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This program shows the structure and replicating processes of DNA and the effect of genetic mutation; demonstrates the Lederberg Experiment; and recapitulates the evidence provided by fossils and structural and biological homologies that the process ...(more details)
 
DVD $99.95
DVD + 3-Year Streaming $149.93
3-Year Streaming $99.95
The Physiology of Exercise

Skeletal muscle exercise expends the stored energy of adenosine triphosphate (ATP) derived initially from the oxidation of glucose. Since most regeneration of ATP for prolonged activity is achieved directly from the oxidation of glucose brought to th...(more details)
 
DVD $69.95
Gel Electrophoresis

This program follows the procedures for placing DNA in a well at one end of an electrophoresis gel: heating the gel and containing it when molten, leaving it to set, loading the digested DNA samples together with loading buffer, and switching on the ...(more details)
 
DVD $79.95
Extraction of DNA from Cells

This program shows the process of DNA extraction: grinding liver tissue using liquid nitrogen to prevent degradation of cellular contents, adding a lysis buffer, rupturing the cell membrane to liberate cellular contents, and separating the DNA from o...(more details)
 
DVD $79.95
Southern Blotting

To enable us to look at specific genes, the DNA is transferred from the gel onto a membrane made of nitrocellulose or nylon. This process is known as southern blotting. The principle is very simple: the capillary action of liquid passing through the ...(more details)
 
DVD $79.95


See additional titles in General Biology | Human Anatomy and Physiology



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